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Description

ME AgarFormula g l Malt Extract 30 Agar 17 Adenine 0. 05 L Histidine 0. 05 L Leucine 0. 05 Uracil 0. 05 Total 47. 20 Suspend 47. 2 gram powdered medium in 1 litre distilled water. Store dry at room temperature.

The cell membranes are discarded

LB Broth Miller is a nutritionally rich medium originally developed for growth and maintenance of recombinant E

: 64485-93-4

R: 36/37/38

35 Suspend 16

MOPS has inert properties and is capable of buffering within a pH range of 6

Cells are grown 1-2 days on pre-sporalation medium before transferring them to sporulation medium

Final concentration is 50mM MOPS

05 L-Lysine HCl 0

Hygromycin B is used as a selective agent in yeasts

98 Agar 7 Final pH 7

5 mM 7

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